Journal: Cell Death Discovery

Article Title: Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome

doi: 10.1038/s41420-020-00293-z

Figure Lengend Snippet: Confocal laser scanning microscopy images of double-label immunofluorescence for CASP-8 (visualized in red fluorescence) and the Nissl-like fluorescent marker Neurotrace (visualized in green fluorescence). a – l Images show the expression levels of the canonical apoptotic marker Caspase 8 in the striatum of 4 and 13 weeks old wild-type mice and in 4 and 13 weeks old R6/2 mice. m , n Bonferroni analysis revealed a significant decrease of striatal cleavage product of Casp-8 positive neurons number, as evaluated by western blotting in 13-week-old Wt and R6/2 mice [genotype effect F 1,36 = 6554; P < 0.05; Time effect F 1,36 = 35,89; P < 0.001].

Article Snippet: Images were acquired by confocal laser scanner microscopy (Zeiss LSM 800) in order to perform soma size analysis.

Techniques: Confocal Laser Scanning Microscopy, Immunofluorescence, Fluorescence, Marker, Expressing, Western Blot

Journal: Cell Death Discovery

Article Title: Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome

doi: 10.1038/s41420-020-00293-z

Figure Lengend Snippet: Confocal microscopy-acquired images of double-label immunofluorescence for Calbindin and NLRP3. Calbindin is showed in green fluorescence, NLRP3 is labelled in red. a – l Images show the immunoreaction intensity of Calbindin and NLRP3 in each experimental group. Calbindin positive neurons and intensity were dramatically decreased in projection neurons of 13 weeks old R6/2 mice F 1,36 = 27,57; p < 0.001. m , n Two way ANOVA analysis performed on data obtained by 4 and 13 weeks old Wt and 4 and 13 weeks old R6/2 mice showed a statistically significant effect of Genotype ( F 1,36 = 108,9; P < 0.001), Time ( F 1,36 = 20.00; P < 0.001) and GenotypeXTime Interection ( F 1,36 = 268,9; P < 0.001) on NLRP3 positive striatal neurons. o Quantitative analysis of coexpression levels by Mander’s coefficient in all experimental groups. Histogram represented the mean of ROIs calculated Mander’s coefficients revealed the statistically significant colocalization of NLRP3 in Calbindin positive neurons P < 0.001.

Article Snippet: Images were acquired by confocal laser scanner microscopy (Zeiss LSM 800) in order to perform soma size analysis.

Techniques: Confocal Microscopy, Immunofluorescence, Fluorescence